KAPA Hyper Prep and HiFi Uracil+ Kits Allow for Reduced Representation Bisulfite Sequencing of Ultra-low Amounts of DNA in B Cells

This application note provides a robust methodology for RRBS analysis using the streamlined chemistry of the KAPA Hyper Prep Kit and HiFi Uracil+ HotStart DNA Polymerase, and demonstrates its utility in accurately quantifying the DNA methylation status from sample-limiting conditions. Methyl-Seq is a powerful tool for understanding genome-wide methylation with base-pair resolution. Several strategies have been developed to understand the methylation status of a genome, including reduced representation bisulfite sequencing (RRBS). Bisulfite treatment results in a significant decrease in DNA input and quality; therefore, the construction of high-quality libraries and efficient downstream amplification is critical.