KAPA2G™ Fast HotStart Multiplex PCR

This application note demonstrates that the KAPA2G Fast HotStart offers faster and more efficient Multiplex PCR than competitor enzymes based on wild-type Taq. KAPA2G Fast HotStart DNA Polymerase is an engineered DNA Polymerase designed specifically for high performance Fast PCR. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the initial denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency. Together with a uniquely formulated buffer, KAPA2G Fast HotStart is the enzyme of choice for Multiplex PCR.