Key considerations in qPCR assay controls

Quantitative PCR (qPCR) is a widely utilized technique for the semi-quantitative study of gene expression. However, its accuracy and reproducibility depend significantly on the proper use of assay controls.

This eBook explores the distinct types of assay controls crucial for reliable qPCR results and provides guidance on the design and application of these controls, including the DNA contamination control, positive PCR control, RNA quality assessment, reverse transcription control, and on the selection and validation of reference genes with the use of prevalidated assays.