Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular interactions critical to mapping the interactome as well as drug discovery efforts. Due to their integration into a lipid bilayer, in vitro characterization of molecular interactions of membrane proteins presents a unique challenge compared to soluble proteins. This application note demonstrates how capturing 150 nm sized lipoparticles that contain a targeted membrane protein on the surface of a biosensor using ForteBio’s octet platform enables the kinetic analysis (k_on, k_diss and K_d) of membrane protein-analyte interactions.