Label-Free Detection of Compound Aggregation Using Corning® Epic® Technology

Small molecule compound aggregation is a major problem encountered in primary screening assays. The Corning Epic technology can detect molecular interaction using optical biosensors integrated into the bottom of Epic microplate wells. Binding or settling of compound aggregates to the biosensor can be detected by measuring the wavelength shift of reflected resonance light. These data demonstrate that Epic technology is ideally suited for the rapid identification of aggregators, and can be readily implemented to attenuate false positives from HTS hit pools.