Label-free protein quantification with 280 nm absorbance measurements

In this application note, Tecan describes a method to quantify proteins in low volumes using the Spark Multimode Reader and the NanoQuant Plate, with UV absorbance measurements at 280 nm. Tecan explains the benefits and limitations of different protein quantification methods and highlights the advantages of using the method. The note also includes step-by-step procedures for using the method and presents performance evaluation results, including detection limit and measurement uniformity.