Large Scale Cultivation of CHO Cells in the Stirred Single-Use Bioreactor BIOSTAT CultiBag STR

The BIOSTAT CultiBag STR is the only single-use bioreactor which is engineering completely on principles known from reusable bioreactors. It is completely comparable to conventional stainless steel bioreactors and fully scalable. In this study, we have demonstrated the successful batch cultivation of CHO DG44 in serum free, chemically defined media under tight feedback control of pH and DO. Cell densities of 6.3 + 106 cells/mL, which are typical for the particular clone when grown in a bioreactor, could easily be reached while maintaining good viability.