Large Scale Cultivation of CHO Cells in the Stirred Single-use Bioreactor BIOSTATR CultiBag STR

In this application note, Sartorius demonstrate the performance of the BIOSTATR CultiBag STR in the batch mode cultivation of CHO in a culture volume of 200 L. Serum free media was used for the cultivation. The particular CHO clone used for this study is the in house generated DG44 ST1-C6 expressing a human IgG. Typically, it reaches cell densities between 6-7 + 106 cells/mL when grown in bioreactors in this type of media.