LC/MS/MS Analysis of Immunosuppressants from Whole Blood using Aeris WIDEPORE XB-C18 Core-Shell HPLC/UHPLC Columns

Cyclosporine A, tacrolimus, sirolimus, and everolimus are four of the most commonly administered immunosuppressant drugs and play a central role in the success of tissue and organ transplants. These drugs are most typically analyzed from whole blood using LC/MS/MS but this presents analytical challenges when working with whole blood. This application note presents a rapid and effective method for the analysis of these four immunosuppressants from whole blood that use a simple protein precipitation step followed by direct injection onto a wide-pore core-shell HPLC column (Aeris WIDEPORE 3.6 μm XB-C18).