<i>De Novo</i> Sequencing Of Genomes Using Up To Five Different Types of Genomic DNA Libraries

Next Generation Sequencing technology enables the de novo sequencing of any kind of genome in a fast and efficient way. This application note describes a strategy which combines sequencing and scaffolding of up to 5 different types of libraries, designed to achieve the optimum result in a reasonable time and at affordable cost. This setup allows us to directly span repeated genetic regions of up to 40 kb.