Light Sheet Microscopy at the Harvard Center for Biological Imaging; Evaluation of the Lightsheet Z.1 Microscope from ZEISS

In the spring of 2013 the Harvard Center for Biological Imaging (HCBI) was able to demo, and eventually acquire, Lightsheet Z.1 microscope from ZEISS. The HCBI have now performed a number of long term embryonic development imaging experiments including imaging of zebrafish, fly, worm, cricket, and salamander embryos. Throughout these experiments, ZEISS Lightsheet Z.1 was employed to acquire both fast dynamics and long-term changes during development; this application note evaluates the use of ZEISS’ Lightsheet Z.1 in such experiments.