Live-Cell Immunocytochemistry

Immunocytochemistry (ICC) is a powerful imaging technique for the visualization of protein location within the cell. However, conventional ICC is not useful for the study of temporal changes in protein expression. This white paper details an integrated solution for real time ICC based on using fluorescently labelled antibody fragments in concert with the IncuCyte S3 Live-cell analysis system. The successful coupling of protein dynamics to cellular morphological changes as well changes in surface expression, cell function and cell-to-cell interactions, are demonstrated.