Loading Capacity for SEC and Reversed Phase HPLC/UHPLC

This application note details the importance of loading capacity in SEC columns when performing protein separations. It illustrates how the high capacity of the bioZen peptide columns, which are comparable to reversed phase HPLC/UHPLC columns, provides good sensitivity - especially in LC-MS peptide separations. The author also discusses the optimum loads in terms of sample volume and sample concentration.