Long Term Culture of Human Mesenchymal Stem Cells (hMSCs) on Corning® Synthemax® II Microcarriers in Xeno-Free Conditions

This application note describes a method developed that allows human bone marrow-derived hMSCs to be cultured in the long term using Corning Synthemax II synthetic microcarriers in xeno-free medium, in disposable spinner flasks. Currently, production of hMSCs for cell therapy faces two challenges: the necessity to grow cells in completely defined conditions (medium and substrate) to avoid any potential contamination and a culture method allowing the generation of very large quantities of cells required for this type of application (up to 5.108 cells/dose).