Looking for a Strong Promoter for <i>Physcomitrella patens</i>

To test promoter strengths, the protoplasts of the moss Physcomitrella patens can be transiently transfected to allow several days of ectopic expression of circular DNA, without the need to produce mutant lines with stable integration. This application note presents a fast, simple, transient assay using BMG LABTECH’s CLARIOstar that can be used to measure GFP and mCherry fluorescence in living protoplasts in a microplate format.