Lower Cost, Higher Throughput Library Preparation with the Echo Liquid Handler and the NuGEN Ovation® Single Cell RNA-Seq System

In this proof of concept study, the Ovation Single Cell RNA-Seq System miniaturization was validated by examining libraries prepared and sequenced at traditional and miniaturized reaction volumes. Additionally, the sample and reagent transfers were automated using the Echo acoustic liquid handling technology. Echo liquid handlers transfer a wide range of fluids without contact of tips or recalibration between fluid types.