mAb titer determination in 60 seconds using the Agilent Bio‑Monolith rProtein A column

This application note tests the recombinant protein A column at the maximum flow rate and performs the bridging study against the native protein A column. A chromatography bind/elute method for mAb titering is demonstrated, which delivers an ultrafast run time (1 minute) suitable for high-throughput applications such as clonal selection, process development, and optimization. In the bridging study, performance characteristics such as retention time, linearity and deviation of the standard curve, sample carryover, and recovery showed no detectable difference between the native and the recombinant columns.