Magnetic Bead Based Soil DNA Isolation Using the Thermo Scientific KingFisher Flex and KingFisher Duo and ClearMag™ Technology

Certain sample materials offer more challenges in DNA purification than the others. Soil samples, for instance, contain lot of PCR inhibitors, which are difficult to remove during sample preparation. The PowerMag™ Soil DNA Isolation Kit was used together with the Thermo Scientific KingFisher Flex and KingFisher Duo magnetic particle processors for DNA purification from several different soil samples. The aim was to optimize an efficient sample preparation system ensuring high yield and quality DNA without inhibitors.