Magnetic Enrichment of TRA-1-60+ Human Pluripotent Stem Cells Facilitates Neural Specification

In this application note from Miltenyi, neural differentiation towards either neural crest (NC) or neural stem cells (NSC) is optimised. A magnetic cell separation protocol to standardise quality and number of true pluripotent stem cells before inducing neural differentiation is developed. The method is based on magnetic enrichment of TRA-1-60+ PSCs that are immediately transferred to neural induction conditions. At the same time, defined cell densities can be adjusted in order to control the differentiation fate towards NSCs or NC cells.