MAGPIX® Bead Loading Optimization

In a Luminex flow cytometer beads are injected single file through a cuvette and are counted and classified as they pass by a laser. When the bead count for all regions reaches the desired value, the system stops acquisition and moves on to the next well. In the MAGPIX system the entire Sample Load Volume is injected into a chamber where the beads are imaged. The image is then processed by the software and the data is presented to the user. This technical note presents a method for optimizing MAGPIX® bead loading.