Maximize Informational Content from Cell-Based Assays Using Multi-Parameter Mechanistic Toxicity Profiling

Testing compounds of interest in the biological context of the cell is a necessary step to identify toxic liability or confirm mechanism of action. This poster shows a mechanistic toxicity profiling panel that has been created in 384-well format consisting of assays used together to evaluate cell viability, cytotoxicity, and apoptosis. The assays consist of detection chemistries for live and dead cell protease activity, ATP content, membrane integrity, and caspase-3/7 activity. Select assays can be multiplexed together to enable more compounds to be analyzed per plate.