Measure cancer cell viability using a homogeneous, stable luminescence assay

Luminescent cell viability assays offer sensitivity and an easy workflow for monitoring the effects of various experimental conditions. These use the firefly luciferase reaction as a way to determine the relative numbers of living cells under different treatments or experimental conditions. Metabolically active cells produce ATP, which is required by the luciferase reaction. When ATP is the limiting component in the reaction, the amount of light produced is proportional to and serves as the readout for the number of viable cells. In this application note, explore how Molecular Devices validated the performance of Promega’s CellTiter-Glo^® 2.0 Cell Viability Assay on the SpectraMax^® iD5 Multi-Mode Microplate Reader.