Measurement of Reactive Oxygen Species in Arabidopsis and N.benthamiana Plants

This application note reports the measurement of transient production of reactive oxygen species (ROS) in response to pathogen attacks using a luminescence coupled assay. The generation of ROS is a typical reaction of plants in response to pathogen infections. The measurement of ROS induced by pathogen-associated molecular patterns (PAMPs) is a valuable tool to identify and characterize PAMPs, to analyze mutants with regard to their immune system and to transfer the knowledge from Arabidopsis to other plant species. The luminescence assay paired with the Berthold Mithras LB 940 multimode reader allows for a quantitative determination of ROS by performing a redox-reaction. These measurements can serve as a robust output of pathogen induced signaling in Arabidopsis as well as in N. benthamiana leaves.