Measurement of the Reduction of Potassium Ferricyanide by L-Ascobic Acid with a Shimadzu UV-1700 Spectrophotometer using a Stopped-Flow Accesory

This application note describes the performance testing of kinetic instruments specifically the Shimadzu UV-1700 with the stopped-flow accessory, SFA-20. The testing is carried out by using the reduction of Potassium Ferricyanide by Ascorbic Acid. This is a well known kinetic reaction and as the speed of this reaction is dependent on the pH value of the solution it makes this a very useful one for testing the performance of kinetic instruments.