Measuring Marker Expression with Imaging Cytometry on a Plate Reader

The presence or absence of cellular proteins can indicate a reaction to stimulation, a state of differentiation, unique characteristics of a specific cell type, or success of a gene transfection. For example, the surface expression of Vascular Cell Adhesion Molecule-1 (VCAM-1) is usually absent from normal vascular endothelium but can be stimulated in vitro by the cytokine TNF-a to elicit an inflammatory response. In this application note read how such protein expressions in a cell can be readily visualized and easily quantified using imaging cytometry.