Measuring Mitochondrial Function and Glycolytic Flux in 3D Cell Cultures

One of the most informative ways to increase the biological relevance of an in vitro assay using 3D cell culture is to measure cell metabolism, whereby perturbed metabolism or mitochondrial function is probed without disrupting the 3D structure. This application note describes use of the FLUOstar Omega microplate reader from BMG LABTECH for the sensitive and high throughput measurement of oxygen consumption and extracellular acidification in 3D cell cultures.