Measuring the DNA damage response in cancer cells using the Spark Cyto multimode reader

The genomic integrity of mammalian cells is constantly challenged by DNA damage arising from both endogenous and exogenous sources. Complex DNA repair pathways have evolved to deal with specific types of DNA lesions. In this application note from Tecan, explore how live cell imaging allows the study of the dynamics of fluorescently tagged repair proteins in real time, and provides the ability to follow cell proliferation changes in response to DNA damaging agents.