Measuring the Interaction of Maltose Binding Protein with Maltose Using MicroScale Thermophoresis

This application note investigates the interaction of maltose binding protein (MBP) and its substrate, maltose, drawing upon label-free MicroScale Thermophoresis (MST) as the ideal technique. MST is a powerful technique to study biomolecular interactions in solution. MST makes use of thermophoresis, the phenomenon of molecules migrating in a solution when a temperature gradient is applied. MST showed sensitivity and convenience, whilst being truly label-free experiments in a close-to-native environment. Additionally, MST is not limited by sample viscosity, further proving its versatility.