Measuring the Output of the CytoTox-Fluor™ Cytotoxicity Assay on the GloMax® Discover System

This application note describes the protocol for measuring fluorescence using the GloMax® Discover System with the CytoTox-Fluor™ Cytotoxicity Assay. The GloMax® Discover System in combination with the CytoTox-Fluor™ Cytotoxicity Assay provides a convenient, rapid and sensitive procedure for determining the number of dead cells in cell populations. Fluorescence correlates with the presence of a distinct protease activity associated with cytotoxicity and loss of membrane integrity.