Mercury- and Fluorinert® - free Piezo-Assisted Transfer of ES Cells into Mouse Embryos Using the Eppendorf PiezoXpert®

Mutant mouse lines derived from injection of genetically modified embryonic stem cells are important and very powerful tools to analyze gene function. This application note shows that injection of ES cells into eight-cell stage mouse embryos or morulae can be achieved with great success using the Eppendorf PiezoXpert without mercury or Fluorinert filled injection capillaries. Additional advantages of the Eppendorf PiezoXpert are also discussed.