Mesenchymal Stem Cell Culture in the New Brunswick™ Galaxy® 170 R CO2 Incubator under Hypoxic Conditions

Common laboratory stem cell cultures involve ambient oxygen conditions in contrast to their native environment where they usually reside under hypoxic conditions. Eppendorf CO2 incubators have a wide array of options that allow for oxygen concentration control, which can be used to create a hypoxic internal environment to mimic physiological conditions experienced by stem cells. This application note demonstrates the low O2 capability of the New Brunswick Galaxy 170 R CO2 Incubator in the culture of Human Mesenchymal Stem Cells (hMSCs).