Metabolite Identification of Payload Species of Antibody Drug Conjugates with Noncleavable Linkers using MetabolitePilot™ 2.0 Software and TripleTOF® 6600 System

There are a number of key challenges involved in antibody-drug conjugate (ADC) metabolism studies, including data quality and incomplete metabolite information. These are addressed with the MetabolitePilot 2.0 Software, which is especially designed for ADCs. The software can be integrated with the SIEX TripleTOF 6600 system, and is fully described in this application note.