Metabolites in Human Urine

In this application note, a method is shown that can be used for routine assays of a urinary purine (Hypoxanthine), a bile acid (Chenodeoxycholic Acid), and nutrients (Betaine and Choline) in biological fluids. It is very sensitive due to the high content of the organic component Acetonitrile in the mobile phase and the use of “MS friendly” Formic Acid. Very reproducible with RSD below 0.5% it is also accurate (MW to 3-4 decimal points).