Metabolomic Profiling in Drug Discovery: Understanding the Factors that Influence a Metabolomics Study and Strategies to Reduce Biochemical and Chemical Noise

In this application note, the Q Exactive Focus mass spectrometer provides a precise and robust platform for untargeted metabolomics studies. The use of systematic studies helps to characterize biological noise, while metabolomic prescreening can help identify biological outliers to ensure homogeneity within an entire study. This application note also demonstrates that fasting is a significant variable in model design, and fasting data can help contextualize drug-induced changes in many metabolites.