Metabolomic Profiling of <em>Neurospora crassa</em> Fungus Using HILIC and Reversed-Phase LC-MS

Much is known regarding the genome of Neurospora crassa, specifically in the determination of circadian rhythms. However, little is known regarding how the metabolome changes over the course of the day under control of the circadian clock. Profiling metabolic changes in biological samples can be a complex and tedious task. The use of the high-resolution Ascentis® Express HPLC columns greatly aids in the resolution of components in even the most complex sample matrix.