Metabolomic Workflow Utilizing Rapid Microbore Metabolic Processing (RAMMP) in Conjunction with SONAR

RAMMP studies have shown comparable group discrimination and improved selectivity over conventional UPLC chromatography. This application note demonstrates further improvements to the workflow by coupling RAMMP with a novel DIA method (SONAR), providing highly specific and unbiased two-dimensional metabolomic data.

SONAR is an acquisition technique comprised of a low-resolution quadrupole mass filter, which is scanned repetitively and both precursor and MS-MS data are acquired at spectral rates approaching 2000 spectra/s.