Metabolomics of Broccoli Sprouts Using UPLC with Ion Mobility Enabled LC/MSE and TransOmics Informatics

Cruciferous vegetables, such as broccoli, cabbage, and kale are known to be anti-carcinogenic and possess antioxidant effects. The aim of this work was to study how the complete set of small-molecule metabolites, the “metabolome,” of broccoli sprouts is modulated under different growth conditions. As the metabolome reflects both genetic and environmental components (e.g., light conditions and nutrients), comprehensive metabolite profiles can describe a biological system in sufficient depth to closely reflect the ultimate phenotypes.