Method Development for Rapid SFC Analysis of Vitamin D3 and Related Compounds

By using the advantages of Supercritical Fluid Chromatography (SFC), namely high permeability and diffusibility, generally higher resolution can be achieved by SFC analysis with shorter run times than by HPLC. Base-line separation of Vitamin D₃ (cholecalciferol), three related compounds (pre-cholecalciferol, 5,6-trans-cholecalciferol, tachysterol₃ ) and the antioxidant (α-tocopherol) is achieved using an Alcyon SFC Triart Diol column under SFC conditions with shorter analysis time, which is one quarter the time for the normal phase HPLC method.