Method Development for the Analysis of Endogenous Steroids Using Convergence Chromatography with Mass Spectrometric Detection

Steroid biosynthesis is a complex metabolic pathway utilizing simple precursors to synthesize multiple steroidal forms. Precise and accurate steroid analysis is critical for the development of steroid-based therapeutics. For mass spectrometric analysis of steroids, due to their structural similarity, chromatographic separation of the steroids is essential prior to analysis. This study focuses on the application of convergence chromatography (CC), utilizing liquid CO 2 as the primary mobile phase, for the rapid chromatographic analysis of endogenous steroids.