Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Analysis of vitamin D metabolites has continued to be a topic of interest in recent publications, primarily as biomarkers for possible disease states and vitamin deficiency. There is also an interest in analytical means to differentiate the D2 and D3 forms from the D2 and D3 epimers because of their different degrees of bioactivity. Ascentis® Express F5 gives a fast and efficient analytical method for 25-hydroxyvitamin D and related forms from serum samples.