Microfluidic Perfusion Enables Long-Term Cell Culture, Precise Microenvironment Control and Gene Expression Analysis

In this application note the M04S Microfluidic Plate and CellASIC^® ONIX Microfluidic System are used to demonstrate long-term culture of adherent cells, to create dynamic solution profiles (media switching and spatial gradient), to immunostain cells within the microfluidic chamber, and to analyze gene expression.