Microinjection into Plant Cells of Etiolated Seedlings Using the Eppendorf InjectMan® 4

For several model systems microinjection is an established method to introduce DNA into single cells to generate transient and stable transformants. Not only nucleic acids but also proteins and small effectors can be injected also in combination to analyze e.g. cellular signal transduction events. This application note presents the greater efficacy of Eppendorf’s new micromanipulation system in increasing the survival rate of injected plant cells.