Microinjection into the Gonads of <i>C. elegans</i> Using Eppendorf InjectMan® 4

Microinjection is an established method for generating transgenic C. elegans strains for the study of protein domains and RNA or DNA regulatory elements. Microinjection is an established method for generating transgenic C. elegans strains. This application note describes a microinjection procedure using the Eppendorf InjectMan®4 micromanipulator.