Miniaturized HTRF® Assays Using the Echo® Liquid Handler

Cisbio’s HTRF assay technology has a wide range of applications in cell-based and biochemical assays. These assays are readily adaptable to high-throughput screening in 384-well and 1536-well formats. This application note demonstrated how the Echo liquid handler enables the miniaturization of HTRF assays to 2 µL assay volumes in 1536-well microplates. Reagents and compounds were transferred at 2.5 nL increments, which enabled flexibility in assay setup and reduced reagent costs.