Minimize Loss of Transfected Cells During Luminescence Assays

Cell attachment is considered to be critical for many cell culture applications since it triggers for instance differentiation, metabolic activity and gene expression. Medium changes and washing steps especially in automated culture processing or high content screenings (HCS) can cause remarkable cell loss.. Here the BRANDplates® cellGrade™ plus surface were tested against comparable TC-treated microplates from different well known manufacturers regarding their ability to support cell proliferation and attachment after transfection. In this experiment success of transfection and cell retention during washing steps was followed by measuring luminescence signal intensity generated by luciferase transfected cells.