Minimizing Phospholipid Interference During LC-MS/MS Analysis of Vitamin D Metabolites in Serum for Clinical Research

Interferences by matrix components can be challenging when measuring serum levels of vitamin D metabolites by LC-MS/MS, particularly lysophosphatidylcholines. They are challenging to remove during sample preparation and difficult to separate chromatographically. This white paper describes an approach for method optimization using the Oasis PRiME HLB solid phase extraction plate, from Waters.