Modeling Ischemia-Reperfusion Conditions with the CLARIOstar

The lack of oxygen supply is associated with life-threatening conditions such as stroke, myocardial infarction or renal failure, whereby cells are temporarily deprived of O₂ and ischemia - an essential nutrient. Investigating these pathologies in vitro requires an experimental set-up capable of rapid deoxygenation, rapid reperfusion, and parallel monitoring of critical biological parameters including cellular oxygenation and ROS. Here, we describe a novel ischemia-reperfusion modeling technique using the CLARIOstar^®.