Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader

Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions, with Luciferases being the most common of these.

In this application note, Molecular Devices demonstrates how the DuoLuc reporter assay and SpectraMax iD5 reader can be utilized to detect nuclear factor-κB (NF-κB) activation in mammalian cell models.