Monitoring Cytotoxicity by Oridonin in U-2 OS Cells in Real Time

This application note describes the use of the BioSpa™ 8 to automate the assay workflow of a kinetic live cell assay using fluorescent probes to monitor cytotoxicity with an automated digital fluorescence microscope. The imaging and analysis of fluorescently stained live cells has traditionally been accomplished using complete manual microscopic methods with exceptionally low numbers of samples. The analysis of higher sample numbers required the use of very expensive dedicated microscopes.