Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP Laboratory Automation Workstation

The well-documented ability of stem cells to differentiate into various cell lineages generates tremendous potential for cell-based treatments. Flow cytometry is a common method for detection of various cell types, but requires automated sample preparation for use in higher throughput situations. This application note demonstrates how the Biomek NX^P Span-8 Automation Workstation can be used for flow cytometry sample preparation to determine the efficiency of cardiomyocyte differentiation from murine embryonic stem cells. The workflow includes fixation, permeabilization, blocking, and antibody staining in a 96-well plate format.